On the Origin of Tepees by Jonnie Hughes

By Jonnie Hughes

All through historical past, we people have prided ourselves on our potential to have rules, yet possibly this satisfaction is lost. might be rules have us. during this booklet, technology author and documentary filmmaker Jonnie Hughes investigates the evolution of rules, looking at how they appear to have lives in their personal. Adopting the position of a cultural Charles Darwin, Hughes travels around the Midwest along with his brother to watch firsthand the average background of ideas--the styles in their version, inheritance, and choice within the cultural panorama. as opposed to Darwin's oceanic islands, Hughes visits the "mind islands" of local American tribes. rather than finches, Hughes searches for indicators of usual choice one of the tepees. With a knack for locating the humor within the quirks of the yank cultural panorama, Hughes takes us on a travel from the Mall of the United States in Minneapolis to what he calls the "maul" of America--Custer's final stand--stopping at roadsides and discoursing on sandwiches, the form of cowboy hats, the evolution of barn roofs, and extra. unique, witty, and fascinating, "On the starting place of Tepees "offers a clean method of realizing either our principles and ourselves.

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6-1: Verwendete Chemikalien Reagenz Tris EDTA NaCL SDS HCL Proteinase K Phenol Chlorophorm Ethanol Agarose: Sea Kem LE Leiter Glycerol Bromphenolblau Xylencyanol Ethidiumbromid Beads Wasser Standard für DNA-Sequenzierers Formamid RAPD-Primer Emzyme Taq, PCR-Puffer, BSA dNTP-Set T4 DNA Ligase, T4 DNA Ligase Puffer AFLP-Adapteren AFLP-Primer Hersteller Roth, Karlsruhe Roth, Karlsruhe Roth, Karlsruhe Roth, Karlsruhe Merk, Darmstadt Appligene, Illkirch Roth, Karlsruhe Roth, Karlsruhe Roth, Karlsruhe Biozym, Hessisch Oldendorf Invitrogen, Carlsbad Merk, Darmstadt Merk, Darmstadt Merk, Darmstadt Serva, Heidelberg Amersham Biosciences: PuRe Taq Ready-ToGo PCR Beads, Piscataway Delta Select: qua ad injectabilia, Dreieich Applied Biosystems: ROX 500 Standard, Forster City Sigma, Deisenhofen MWG Biotech, Ebersberg NEB, Ipswich Qiagen, Hilden Qiagen, Hilden NEB, Ipswich MWG Biotech, Ebersberg MWG Biotech, Ebersberg 22 Material und Methoden Für die DNA-Isolierung und die Gelelektrophorese wurden Puffer in folgender Zusammensetzung eingesetzt: Gewebelysispuffer: 10 mM Tris-HCL pH 7,5, 1 mM EDTA pH 8,0, 5 mM NaCl, 10 % SDS 10x TE Puffer: 100 mM Tris-HCL, 10 mM EDTA pH 8,0 10x TBE Puffer: 890 mM Tris-HCL, 890 mM Borsäure, 25 mM EDTA pH 8,3 Ladepuffer: 50 % Glycerol, 50 % 1x TBE, 0,25 % Bromphenolblau, 0,25 % Xylencyanol Als DNA-Leiter wurde verwendet: 1000 μl Invitrogen Stock Loading dye 6x , 8000 μl 1x TE pH Das für die Gelelektrophorese und den auf 1x verdünnten TBE-Puffer eingesetzte Wasser war ultrafiltriert und entmineralisiert.

Dies gelang nicht mit der Population Ossenfeld II oder etwa mit der Population Oberkatzbach (durchschnittlich 48 Eier), in der die erste Filialgeneration erst drei Jahre nach der Eiablage aufgezogen werden konnte. 47 Ergebnisse Im Mittel abgelegte Eier 120 100 80 60 40 20 Oberkatzbach Moosach Ebersberg Weiher Ebersberg Weg Weißwassertal Scheden Ossenfeld II Ossenfeld I Zwölfgehren Ludolfshausen Knutbühren Kaufunger Wald Hildesheim Hasbruch Groß-Ellershausen Göttinger Wald Bremke Bad Lauterberg Bad Laer 0 Population Abb.

Hierzu wurden 1052 μl Formamid mit 48 μl Rox-500-Standard (Fa. Applied Biosystems) gemischt. Der Ansatz ist ausreichend, um in jeder Vertiefung der Trägerplatte 12,5 μl aufzunehmen. Von dem Amplifikat wurden nun dem Lösungsmedium zunächst 1,5-2,5 μl hinzugegeben und mit der Pipette vermischt. Nachdem alle Proben aufgebracht waren, wurde die Platte kurz zentrifugiert. Dann wurden die Proben zwei min bei 94 °C in einem Thermocycler (T Gradient, Fa. Biometra) denaturiert und sofort auf Eis abgekühlt, um ein erneutes Zusammenlagern der nun einzelsträngig vorliegenden DNA zu verhindern.

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