By R.C. Arridge, P.J. Barham, M. Kawaguchi, J. Kolarik, A. Takuhashi
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Both samples were purified by HPLC prior to FDMS. ; ACS Symposium Series; American Chemical Society: Washington, DC, 1980. CELL SURFACE GLYCOLIPIDS Figure 8. Field desorption mass spectrum obtained at 22 ma for a mixture of cerebrosides from bovine brain. Assignments of MH* are discussed in the text and summarized in Table 1. ; ACS Symposium Series; American Chemical Society: Washington, DC, 1980. 3. Field Desorption COSTELLO ET A L . Mass Spectrometry 43 of bovine c e r e b r o s i d e s . Assignment of the s t r u c t u r e s to the FD peaks was made under the assumption t h a t the sample c o n s i s t e d of a mixture of cerebrosides v a r y i n g i n the nature of t h e i r s i d e chains and t h a t each cerebroside gave r i s e to a protonated molec u l a r i o n .
Figure 14 shows the spectra of t h i s d e r i v a t i v e obtained at 14 and 22 ma. At the higher temperatures, the spectrum had many s i g n i f i c a n t fragments, but none corresponding to Cleavage A i n Scheme A . The t r i f l u o r o a c e t y l d e r i v a t i v e showed a weak, unstable c l u s t e r of ions centered at the MH ion (m/e 1682). I t s spectrum was dominated by consecutive losses of CF3CO (mass 97), so t h a t i t appears to be an a n a l y t i c a l l y l e s s useful d e r i v a t i v e .
4. 3) a 6 (J 1 —MGal 3 —•ICer ) Gal VI 57 Spectra 1 _^3Gal 1 — • 3GalNAc 6 ( NMR Coupling Constants o f G l y c o s p h i n g o l i p i d s i n DMS0-d5. GalNAc V Proton DABROWSKI ET A L . 2 3 3 3 3 Gal l - * . ; ACS Symposium Series; American Chemical Society: Washington, DC, 1980. ; ACS Symposium Series; American Chemical Society: Washington, DC, 1980. 3 1-^lCer 4. DABROWSKI ET A L . Figure 1. 360-MHz Figure 2. Proton NMR 59 Spectra proton NMR spectrum of glucosylceramide in For other conditions, see Methods.